pAVD10 contains the uncF gene (encoding the beta-subunit of the E. coli ATPase) under the control of the bacteriophage T7 promoter. It is used to verify the identity of the strains C41(DE3) and C43(DE3).
pAVD10 Entire Sequence (3031 pb) 1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat 60 61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 120 121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 180 181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 240 241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 300 301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 360 361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 420 421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 480 481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 540 541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 600 601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 660 661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 720 721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact 780 781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 840 841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 900 901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 960 961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1020 1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1080 1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1140 1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1200 1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1260 1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1320 1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1380 1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1440 1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1500 1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1560 1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1620 1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1680 1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 1740 1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 1800 1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 1860 1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 1920 1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 1980 1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2040 2041 tctgtgcggt atttcacacc ggtgcactct cagtacaatc tgctctgatg ccgcatagtt 2100 2101 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg 2160 2161 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa 2220 2221 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc 2280 2281 gcgaggCAGG ATCTCGATCC CGCGAAATTA ATACGACTCA CTATAGGGAG ACCACAACGG 2340 2341 TTTCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACATAT GAATCTTAAC 2400 2401 GCAACAATCC TCGGCCAGGC CATCGCGTTT GTCCTGTTCG TTCTGTTCTG CATGAAGTAC 2460 2461 GTATGGCCGC CATTAATGGC AGCCATCGAA AAACGTCAAA AAGAAATTGC TGACGGCCTT 2520 2521 GCTTCCGCAG AACGAGCACA TAAGGACCTT GACCTTGCAA AGGCCAGCGC GACCGACCAG 2580 2581 CTGAAAAAAG CGAAAGCGGA AGCCCAGGTA ATCATCGAGC AGGCGAACAA ACGCCGCTCG 2640 2641 CAGATTCTGG ACGAAGCGAA AGCTGAGGCA GAACAGGAAC GTACTAAAAT CGTGGCCCAG 2700 2701 GCGCAGGCGG AAATTGAAGC CGAGCGTAAA CGTGCCCGTG AAGAGCTGCG TAAGCAAGTT 2760 2761 GCTATCCTGG CTGTTGCTGG CGCCGAGAAG ATCATCGAAC GTTCCGTGGA TGAAGCTGCT 2820 2821 AACAGCGACA TCGTGGATAA ACTTGTCGCT GAACTGTAAT AAAAGCTTAG GCCTCTAGTC 2880 2881 TAGACTAGAA TTCCGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG 2940 2941 CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT 3000 3001 TTTTGCTGAA AGGAGGAACT ATATCCGATA A
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