The procedure is as follows:.

On Day 1 :
• Thaw an aliquot of competent cells on ice for 10 minutes
• Add to the competent cells 1µl of pAVD10 plasmid (5ng/µl) on ice for 20 minutes .
(the cells are fragile: mix gently, but do not pipette them)
• Heat-shock the transformation reaction in a 42°C water bath for 2 minutes.
• Incubate the reactions on ice for 3 minutes.
• Add 450 µl of LB medium to each transformation, and incubate the reactions at 37°C for 1 hour, with shaking, at 200 rpm.
• Plate:
       - 100 µl of the transformation reaction (using a sterile spreader) onto a LB + ampicillin plate and
       - 100 µl onto a LB + ampicillin + IPTG plate.
• Incubate the plates overnight at 37°C.

On Day 2 :
Count the number of colonies on the LB-ampicillin and LB-ampicillin-IPTG plates.

Expected Results :